The phagocyte NADPH oxidase depends on cholesterol-enriched membrane microdomains for assembly
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منابع مشابه
The phagocyte NADPH oxidase depends on cholesterol-enriched membrane microdomains for assembly.
The superoxide-producing phagocyte NADPH oxidase consists of a membrane-bound flavocytochrome b558 complex, and cytosolic factors p47phox, p67phox and the small GTPase Rac, which translocate to the membrane to assemble the active complex following cell activation. We here show that insolubility of NADPH oxidase subunits in nonionic detergents TX-100, Brij-58, and Brij-98 is a consequence of inc...
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F ~hagocytic cells contain a complicated enzyme system, termed NADPH oxidase, that is responsible for the production of toxic oxygen species (1). The enzyme transfers electrons from NADPH to 02, forming O2-, which then can dismutate into H202. Subsequently, other oxygen derivatives, such as hydroxyl radical and hypochlorous acid, may be formed. Collectively, these oxygen products are toxic to c...
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In this issue of Blood, Zhang et al show that mice lacking the stromal-interacting molecule 1 (STIM1) gene in bone marrow cells are more susceptible to bacterial infection but are resistant to ischemia/reperfusion injury because of defective activation of phagocyte nicotinamide adenine dinucleotide phosphate (NADPH) oxidase.
متن کاملThe phagocyte NADPH oxidase and bacterial infections
Neutrophils play a crucial role in host defense against microbial infections. During phagocytosis of invading bacteria or fungi, the phagocyte NADPH oxidase produces superoxide. The importance of the oxidase is exemplified by the genetic disorder known as chronic granulomatous disease (CGD). The neutrophils of CGD patients cannot produce superoxide, with the result that affected infants and chi...
متن کاملAssembly of the phagocyte NADPH oxidase: molecular interaction of oxidase proteins.
Phagocytes such as neutrophils play a key role in the body's innate immune response to infection. These cells travel throughout the body in search of pathogens and are rapidly mobilized to sites of inflammation where they phagocytose these pathogens and subsequently release a variety of toxic oxygen radical species and proteolytic enzymes to directly destroy the engulfed particle. The generatio...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2004
ISSN: 0261-4189,1460-2075
DOI: 10.1038/sj.emboj.7600066